Course Syllabus

Export

Il corso si propone di fornire allo studente i principi generali che permettono di comprendere quali informazioni possono essere ottenute mediante le principali tecniche di analisi per la caratterizzazione di nanobiomateriali. 

Conoscere i principi di funzionamento delle piu’ importanti tecniche e dei piu’ diffusi strumenti di analisi biofisica utilizzati per lo studio delle caratteristiche di nanoparticelle e nanomateriali di interesse biomedico.

1) Introduzione alle tecniche ottiche • Spettri di assorbimento ed emissione • Spettrofotometro e coefficiente di assorbimento • Attivita’ ottica (Optical Rotatory Dispersion, ORD) • Dicroismo circolare (CD) e Birifrangenza ottica 

2) Tecniche di Fluorescenza • Fluorescenza di aminoacidi, acidi nucleici e altre biomolecole • Fluorescenza risolta in spettro e risolta in tempo • Spettrofluorimetro • Fluorofori in microscopia. • Polarizzazione e anisotropia di fluorescenza. 

3) Tecniche di microscopia ottica • Tecniche avanzate di imaging • Microscopio a contrasto di fase • Microscopio a fluorescenza • Microscopio polarizzatore • DIC (Differential Interference Contrast) 

4) Tecniche avanzate di microscopia quantitativa • Microscopia confocale • FRET (Fluorescence Resonance Energy Transfer) • FCS (Fluorescence Correlation Spectroscopy) • TIRF (Total Internal Reflection Fluorescence) • FRAP (Fluorescence Recovery After Photobleaching) 

5) Tecniche di microscopia non ottica • AFM (Atomic Force Microscopy) • Microscopio elettronico: SEM (Scanning Electron Microscopy), TEM (Trasmission Electron Microscopy) 

6) Tecniche di nanomanipolazione • Magnetic Tweezers (MT) e Optical Tweezers (OT) 

7) Light Scattering • Static Light Scattering (SLS) • Dynamic Light Scattering (DLS) • Z-potential 

8) Altre tecniche di analisi rilevanti per le nanobiotecnologie • Spettroscopia Raman • SERS (Surface Enhanced Raman Spectroscopy) • SPR (Surface Plasmon Resonance) • ITC (Isothermal Tritration Calorimetry) e DSC (Differential Scanning Calorimetry) • FTIR (Fourier Transform Infrared Spectroscopy) 


Conoscenze di base di chimica, biochimica e biologia molecolare  


Lezioni frontali e esercitazioni. 

Nel periodo di emergenza Covid-19 le lezioni si svolgeranno in modalità mista: parziale presenza e lezioni videoregistrate asincrone/sincrone con alcuni eventi in presenza fisica.


Materiale e riferimenti bibliografici forniti dal docente.

N. R. Zaccai, I. N. Serdyuk, J. Zaccai, “Methods in Molecular Biophysics: Structure, Dynamics, Function for Biology and Medicine”; Editor: Cambridge University Press; ISBN-13: 978-1107056374

Y. L. Lyubchenko, "An Introduction to Single Molecule Biophysics", Editor: CRC-Press; ISBN-13: 978-1351333122

D. E. Makarov, "Single Molecule Science: Physical Principles and Models", Editor CRC Press; ISBN-13 : 978-0367575717




Primo semestre

Esame orale: approfondimento su articolo scientifico preventivamente fornito allo studente qualche settimana prima dell’esame.

Esame scritto: Domande a risposta multipla a carattere generale sugli argomenti svolti a lezione.

Nel periodo di emergenza Covid-19 gli esami saranno solo telematici. Verranno svolti utilizzando la piattaforma WebEx e nella pagina e-learning dell'insegnamento e verrà inviato agli studenti un link per l'accesso all'esame.

Su appuntamento telefonico (02 6448 8209) o via mail (francesco.mantegazza@unimib.it). 


Export

The course aims to provide the students with the knowledge on the general principles enabling to understand which kind of information can be achieved by using the most important analysis techniques for nanobiomaterials characterization.

To learn the working principles of the most important analytical techniques and of the most relevant instrumentations used for the characterization of nanoparticles and nanomaterial of biomedical interest.

1) Introduction to optical techniques • Spectra of absorption and emission • Spectrophotometer and the absorption coefficient • Optical Activity (Optical Rotatory Dispersion, ORD) • Circular dichroism (CD) and optical birefringence 

2) Fluorescence techniques • Fluorescence of amino acids, nucleic acids and other biomolecules • Resolved fluorescence spectrum and time-resolved • Spectrofluorimeter • Fluorophores microscopy. • Polarization and Anisotropy of fluorescence. 

3) Optical microscopy techniques • Advanced Imaging Techniques • Phase contrast microscope • Fluorescence microscope • Polarizing Microscope • DIC (Differential Interference Contrast) 

4) Advanced quantitative microscopy techniques • Confocal Microscopy • FRET (Fluorescence Resonance Energy Transfer) • FCS (Fluorescence Correlation Spectroscopy) • TIRF (Total Internal Reflection Fluorescence) • FRAP (Fluorescence Recovery After Photobleaching) 

5) Non optical microscopy techniques • AFM (Atomic Force Microscopy) • Electron Microscope: SEM (Scanning Electron Microscopy), TEM (Transmission Electron Microscopy) 

6) Nanomanipulation techniques • Magnetic Tweezers (MT) and Optical Tweezers (OT) 

7) Light Scattering (LS) • Static Light Scattering (SLS) • Dynamic Light Scattering (DLS) • Z-potential 

8) Other relevant analysis techniques for the nanobiotechnology • Raman Spectroscopy • SERS (Surface Enhanced Raman Spectroscopy) • SPR (Surface Plasmon Resonance) • ITC (Isothermal Calorimetry Tritration) and DSC (Differential Scanning Calorimetry) • FTIR (Fourier Transform Infrared Spectroscopy) 

Basic knowledge in chemistry, biochemistry and molecular biology 


Lectures and exercises.

During the Covid-19 emergency period, lessons will take place in a mixed mode: partial presence and asynchronous / synchronous videotaped lessons with some physical presence events.

 


Material and bibliographic references supplied by the professor.

N. R. Zaccai, I. N. Serdyuk, J. Zaccai, “Methods in Molecular Biophysics: Structure, Dynamics, Function for Biology and Medicine”; Editore: Cambridge University Press; ISBN-13: 978-1107056374

Y. L. Lyubchenko, "An Introduction to Single Molecule Biophysics", Editor: CRC-Press; ISBN-13: 978-135133312


D. E. Makarov, "Single Molecule Science: Physical Principles and Models", Editor CRC Press; ISBN-13 : 978-0367575717

First semester

Oral examination: discussion about a scientific paper previously provided to the student some weeks before the date of the exam.

Written exam: General multiple choice questions about the topics afforded during the lessons.

In the Covid-19 emergency period, exams will be online only. They will be conducted using the WebEx platform and on the e-learning page of the course and a link will be sent to the students to access the exam.

By telephone appointment (02 6448 8209) or by email (francesco.mantegazza@unimib.it).

Enter

Key information

Field of research
NN
ECTS
6
Term
First semester
Activity type
Optional
Course Length (Hours)
48
Language
English

Staff

    Teacher

  • Francesco Mantegazza
    Francesco Mantegazza
  • DS
    Domenico Salerno

Students' opinion

View previous A.Y. opinion

Bibliography

Find the books for this course in the Library

Enrolment methods

Manual enrolments
Self enrolment (Student)