The 1982 UN Convention on the Law of the Sea define Marine pollution as: “the introduction by man, directly or indirectly, of substances or energy into the marine environment … which results or is likely to result in such deleterious effects as harm to living resources and marine life. Since marine pollution can change the physical, chemical, and biological state of our oceans, is is a severe threat to marine wildlife and ecosystems, and the industries and livelihoods dependent on them, such as fisheries and tourism. Moreover, toxic chemicals that become concentrated in the food chain may impact human health.The UN’s Sustainable Development Goal target 14.1 indicates: ‘’By 2025, prevent and significantly reduce marine pollution of all kinds, in particular from land-based activities, including marine debris and nutrient pollution. The course presenst the state of the art regarding the available technologies for the determination of the contamination of the marine environment and the definition of marine pollution. Specifically the basis of the environmetal analysis from sampling to sample preparation and detection, the application of non destructive technicques such as UV-VIS, IR, XRF and raman spectroscopy, iphenated techniques such as LC-MS and GC-MS, and recente advances for underwater chemical analysis. The course include also laboratory experience with infrared spectroscopy and mass spectrometry